The Importance of Proper Timing

Timing is the easiest factor to fix in fertility treatment, but identifying your fertile window is not always as straightforward forward as counting days - especially when there are factors such as excess inflammation, pathological accumulation of fluids in your reproductive system, and impairments to ideal hormone signaling at play.

Algorithmic apps that make predictions based on previous cycles are not accurate or reliable for predicting ovulation 🙅‍♀️. Every cycle is independent from the rest and influenced by a multitude of factors.

The Six Day Fertility Window

There are only six days maximum out of your cycle when sex can result in pregnancy: the five days proceeding ovulation and the day of ovulation itself. The factors that influence this time frame include the viability of your egg and the ability of sperm to survive within your reproductive tract.

Egg Viability:

Once ovulation occurs, your egg remains viable for only 12-24 hours, creating a brief window for fertilization. Ideally, healthy sperm should already be waiting within your fallopian tubes when ovulation occurs - like Times Square gatherers on NYE waiting for the ball to drop.

Sperm Survival:

Upon entry into your reproductive tract, sperm can survive for up to 5 days waiting for an egg to be released. Factors that can influence how long sperm actually survive include:

pH and Microbiome

Unlike the microbiome of your digestive tract which is made up of a diverse array of bacterium species, the microbiome of your reproductive system is composed primarily of lactobacillus species. These bacteria help maintain a naturally acidic environment that helps protect against infectious bacteria. When out of balance, local inflammation and increased immune activity can damage sperm viability as they await their big moment.

Cervical Fluid

When optimized, the fluid produced by your cervix in response to the rising estrogen levels produced by your growing egg follicles sorts out imperfect sperm, aids in the transport of healthy sperm to the fallopian tubes, protects sperm from the naturally acid environment of your vagina and uterus, and nourishes the sperm as they wait for ovulation to occur.

Immune System Function

Out of balance immune responses may target sperm as unwelcome invaders and initiate a seek and destroy campaign. Increased inflammation from excess local or systemic immune activity can lead to increased free-radical activity that damages both the physical structure of sperm and the vital genetic information that they carry.

Sperm Health

The health of your partners sperm, and quality of the semen in general, will affect their viability within your reproductive system.

Inflammation, imbalanced microbiome, immune dysfunction, and excess free radical activity can significantly impact sperm count, the physical structure and ability of sperm to move forward through your vagina and uterus, and the genetic integrity of the sperm which is required for proper fertilization and early embryonic growth - all before they even leave your partners body.

When these impaired semen parameters are in play, the survival rate of sperm is negatively impacted, leaving less quality sperm available to survive long enough to meet the egg at ovulation.

Tracking Ovulation 📈

Identifying your fertile window by tracking signs of ovulation and hormone levels is essential for timing intercourse and increasing your chances of conception - especially when there are factors at play that may be chipping away at optimal fertile window length.

Methods of Tracking Ovulation

(In order of my most to least favorite)

BBT (Basal Body Temperature) Monitoring:

Tracking your base metabolic activity by taking your temperature first thing in the morning every day with a specialized thermometer (calibrated to normal body temperature, measures to the hundredth of degrees, typically has a memory function) is a time-tested and informative way to gather information, not just about when you’ve ovulated, but your hormonal and metabolic health in general.

Pros of BBT Tracking:

⇒ Low financial investment. Thermometers range from $10 (basic) to $40 (app compatible)

⇒ Confirms ovulation

⇒ Can be used diagnostically by assessing temperature ranges and shifts throughout the cycle allowing for customized protocols

⇒ Usefully in visualizing changes as treatment progresses

Cons of BBT:

⇒ Does not predict when ovulation will occur but can confirm that it took place

⇒ Can lead to over-analysis paralysis. It’s important not to fixate on individual temperatures or focus too hard on trying to shift the temperatures with hacks

⇒ Can be influenced by poor sleep, illness, alcohol consumption

Hormone Monitoring Devices:

The inito hormone monitoring device is a small reader that connects to your phone (make sure yours is compatible) that measures the hormone levels via urine strips. The app will alert you to which days you should test (~15 days per cycle) and then creates a graphic representation of FSH, LH, Estrogen, and Progesterone levels.

Pros of using the inito device:

⇒ Gives real time data on hormone levels and relationships show rising estrogen levels and the LH surge as ovulation approaches to ensure proper timing of trying and confirms ovulation via progesterone levels in the luteal phase

⇒ Can easily be sent to me for review

⇒ HSA/FSA Eligible

⇒ Tracks multiple hormones, not just LH

Cons of using the inito device:

⇒ Initial investment of device $89-149 (use discount code LAURA89)

⇒ Ongoing cost of test strips ($50/cycle)

⇒ Biometric data released into the cloud 🤷‍♀️

⇒ Can lead to over-analysis paralysis. As with other forms of cycle tracking, it’s important not to get hung up on single data points, but observe patterns over time and use the information to fine tune treatment strategies going forward. There are no magical quick fixes when it comes to fertility. Your body is cyclical, your eggs are recruited in waves that started 3 to 4 months prior to your current cycle and there is no beneficial way to rush the process.

Cervical Fluid Monitoring:

The fluid/mucus that your cervix produces throughout your cycle has many roles depending on where you are in your cycle. After your period and before ovulation, your cervical fluid is thick and sticky, effectively closing your cervix against entry of foreign material into your uterus.

As ovulation approaches, in response to rising estrogen levels produced by your growing ovarian follicles, your cervical fluid begins to thin, becoming clear, slippery, and stretchy.

This type of cervical fluid is vital for assisting and protecting sperm as it travels through your reproductive tract into your fallopian tubes to hopefully await an egg at ovulation.

Pros of Cervical Fluid Monitoring

⇒ Free, no special equipment or devices needed

⇒ Obvious when optimized

⇒ If absent, indicates specific imbalances to address

Cons of Cervical Fluid Monitoring

  • Slight ick factor if you’re squeamish about body fluids

  • Does not confirm ovulation only rising levels of estrogen

  • If your estrogen levels are low or other imbalances are at play, your cervical fluid changes may not be obvious

OPK strips:

Ovulation Predictor Kit strips are commonly used in the days leading up to predicted ovulation to identify your LH surge. Tests are interpreted by comparing the darkness of the ‘test’ line (the line that appears when there is significant LH present in the urine sample) to that of the ‘control’ line (which always appears to indicate the test is working). A light line indicates that LH levels are rising, while a line that is the same color or darker than the control line indicates peak LH levels and that ovulation is likely to occur in the next 24-36 hours.

Some newer and more advanced tests may give results with words or symbols, rather than the control and test lines.

Pros of Using OPK Test Strips:

⇒ Easy to use

Cons of Using OPK Test Strips:

⇒ Ongoing cost

⇒ Cannot confirm ovulation, only that there has been a surge of LH released

⇒ Timing is imprecise. It can be challenging to interpret the LH surge, which typically happens 24-36 hours before ovulation.

⇒ Does not assess any other fertility markers

⇒ Can give false positives. Sometimes there is more than one LH surge, especially in an under optimized cycles where the hormonal signaling between the brain and the ovaries is impaired. In conditions such as PCOS, the baseline levels of LH may be higher throughout the month and give false positives even when ovulation doesn’t occur.

Cervical Position and Openness:

Prior to the advent of newer technologies and monitoring devices, assessing ovulation was done primarily with a more hands on in approach.

Throughout your cycle, your cervix changes position and openness. During your period, your cervix is open to allow the discharge of your uterine lining. Following your period, it closes up and becomes sealed with thick, sticky cervical fluid. As ovulation approaches, the cervical reopens and your cervical fluid thins to facilitate the entry of semen into your uterus. Following ovulation, your cervix closes again to protect a potential pregnancy from bacteria or foreign material that may enter the uterus.

These changes can be felt with practice be inserting your finger or fingers into your vagina and feeling the relative position and openness of your cervix.

At ovulation cervix has a softer feel, is higher up within your vaginal canal, is open, and feels wet due to the presence of fertile cervical fluid. The acronym SHOW is used to help remember these qualities ties:

⇒ Soft

⇒ High

⇒ Open

⇒ Wet

Pros of Observing Cervical Position and Openness

⇒ Free and informative

⇒ Fosters autonomy and greater understanding of your body

⇒ Cons of Observing Cervical Position and Openness

⇒ Takes practice to build confidence in your observations

⇒ A little awkward to manage at first

Timing Sex During the Fertile Window

Optimal Timing:

Your most fertile days are the five days before ovulation and the day of ovulation - in an optimized cycle with healthy sperm. When your or your partners hormones are off, this window may be narrower.

Concentrating intercourse during this window increases the likelihood of having sperm ready and waiting in the fallopian tube when ovulation occurs 🪩

Your highest probability days for conception in an optimized cycle are as follows:

⇒ Day of LH surge (day before ovulation) = 30-40% chance of conception

⇒ Day of ovulation = 20% chance of conception

Chances of Pregnancy based on timing (in an optimized cycle with healthy sperm):

⇒ 3 days before ovulation: 27%

⇒ 2 days before ovulation: 33%

⇒ 1 day before ovulation: 41%

⇒ Day of ovulation: 20%

⇒ 1 day after ovulation: 8%

Abstinence v. Frequency of Sex

It’s a common misconception that abstaining from sex until ovulation maximizes sperm concentration and improves chances of conception. However, research findings show that having intercourse every other day leading up to ovulation is more effective than prolonged abstinence

Effects of Frequency:

⇒ Frequent/Daily ejaculation may slightly reduce sperm count and motility

⇒ Prolonged abstinence can lead to decreased sperm quality due to aging of the sperm

⇒ Recommendation: Sex every other day from 5 days before ovulation is expected until ovulation is confirmed via:

  • Upward temperature shift of BBT by approximately 1 degree

  • Rising PdG levels on inito readings

  • No more clear, slippery, stretching cervical fluid is observed

    • This doesn’t 100% confirm ovulation but it does indicate that you are no longer in your fertile window

Following this recommendation ensures a consistent presence of viable sperm in your reproductive tract during the fertile window without compromising quality

Factors Affecting Fertilization 💥

As you can see from the percentage rates of conception listed above, even under the best of circumstances there is never a 100% guarantee for conception in any given cycle. Fertilization success can be influenced by several factors including the timing of sex real active to your fertile window, the ability of sperm and egg to meet within the fallopian tubes, and the quality of the individual sperm and egg involved.

Timing

The timing of sex (as indicated above) can affect the chances of fertilization if the egg and sperm are not synched up to meet in a timely manner. Misjudging your fertile window or relying on an algorithmic app to tell your fertile days can lead to a missed connection.

Ability to Meet Up

Tubal Patency

Tubal patency, or the openness of your fallopian tubes, is a key factor in fertilization. If your tubes are obstructed in any way, the sperm can’t reach your egg before it starts to degrade. Your eggs are only viable for up to 24 hours and travel time through the fallopian tubes to the uterus is 5 to 8 days.

Tubes can be obstructed by several factors:

⇒ Excess mucus and pathogenic fluids may block your tubes preventing sperm from moving through them.

⇒Scar tissue and adhesions can result from chronic inflammation associated with endometriosis, subclinical infections or past infections, or pelvic inflammatory disorder.

Cervical Fluid

Variations in cervical fluid can impact the ability of sperm to swim rapidly to the fallopian tubes. Is there sufficient cervical fluid to transport, nourish, and protect sperm as it travels through the reproductive tract to reach the fallopian tubes?

Genetic Integrity

Your egg and your partner’s sperm must both carry intact DNA for fertilization to occur and early embryonic development to proceed.

High levels of DNA fragmentation in the sperm significantly reduce fertilization rates and embryo viability

These genetic factors are influenced by hormonal, inflammatory, and environmental conditions.

Factors Affecting Successful Implantation 🌱

When sperm and egg can successfully meet up, they are both genetically viable, and fertilization occurs, the ability of the developing embryo to implant into the uterus is the next achievement that needs to occur for pregnancy to occur. Like everything else that we’ve talked about so far, there are multiple factors at play when it comes to the ability of embryo to successfully implant and move forward in its development.

Embryo Health

The quality and chromosomal normalcy of the developing embryo significantly impact its ability to implant successfully. This ability is the direct result of the genetic information contained within the egg and sperm.

Tubal Obstructions

Endometrial receptivity

the receptivity of the uterine lining depends on proper hormonal signaling, particularly adequate progesterone levels

  • Progesterone supply is dependent on both the proper formation and function of the corpus luteum following ovulation and the ability of the uterine lining itself to respond to the progesterone

  • The thickness and quality of the uterine lining are essential for implantation and nourishment of the embryo

    • The growth of the endometrium in the follicular phase is stimulated by proper estrogen levels

Uterine Microbiome

The balance of microorganisms in the uterus can influence implantation success and early pregnancy development

Inflammatory Factors:

Excessive inflammation or the presence of scar tissue can interfere with the implantation process

Overview of Fertilization to Implantation Timeline 🎢

  • Fertilization: once the egg and sperm come together in fertilization within the fallopian tube, the newly formed embryo begins to rapidly divide and develop

    • This process is driven by the quality of the genetic material provided by both sperm and egg

  • Early development: over 5-7 days following fertilization, the embryo develops in the fallopian tube, its cell count increases through rapid cellular division to form a blastocyst with 70-200+ cells while traveling through the tube towards the uterus

  • Implantation: by 7-10 days post ovulation (DPO), the embryo (early blastocyst stage) begins to implant in to the uterine wall initiating early pregnancy

    • Implantation bleeding may occur ~7-8 days after ovulation

      • Spotting for ~1 day